Cryopreservation of human saphenous veins

Prefreezing equilibration with the cryomedium

 Exposure to the DMSO-containing cryomedium for up to 4 h without freezing does not modify contractile force of human saphenous veins.

If, however, equilibration is followed by freezing, maximal contractile responses are significantly diminished. 

 

Cooling rates

Different cooling rates are required for optimal post-thaw functional activity of human saphenous veins (SV) and internal mammary arteries (IMA). Optimal post-thaw recovery is obtained at cooling rates of -0.6 and -1C.

 

Thawing speed 

Slow warming improves slightly the post-thaw contractile function 

 

 

Long-term storage 

Suspended in RPMI containing 1.8 M DMSO plus 0.1 M sucrose human saphenous veins may survive for years when stored in liquid nitrogen at -196 C [25].

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