Activation of

protein kinase C

 

In cryostored human IMAs contractile responses to ET-1 are enhanced. This appears to be the due to activation of the protein kinase C and can be reversed by  staurosporine [23]

 

Left: Cumulative concentration- response curves to ET-1 on unfrozen and cryostored human IMA. Effects are expressed in g.

Right: Contractile responses to a single concentration of 100 nM ET-1 of unfrozen and cryostored human IMA. Effects are expressed in g.


 

 

 

 

 

 

 

Blockade of protein kinase C with 100 nM staurosporine reversed  the longlasting tonic contraction of cryostored human IMA in response to a single addition of ET-1.



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